The observation of a hedamycin‐d(CACGTG)2 covalent adduct by electrospray mass spectrometry
Authors:
- P IannittiPIP Iannitti
- J BoschenokJBJ Boschenok
- M M SheilMSM M Sheil
- 6 March 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 360 (3) , 231-234
Abstract
Covalent binding of the antitumour antibiotic hedamycin to the self‐complementary hexadeoxyribonucleotide 5′‐CACGTG‐3′ has been investigated by electrospray ionization mass spectrometry (ESI‐MS). Ions due to double‐stranded forms of the free 5′‐CACGTG‐3′ and the hedamycin‐5′‐CACGTG‐3′ adduct have been observed in ESI mass spectra and their identity has been confirmed by resolution of individual charge states in ESI‐MS spectra. Clear evidence that specific base‐paired associations are being observed in ESI‐MS is provided by the results of a titration experiment involving alkylated and non‐alkylated complementary strands. This work demonstrates the potential of this powerful new tool for studying ligand‐DNA binding.Keywords
This publication has 7 references indexed in Scilit:
- Observation of Duplex DNA-Drug Noncovalent Complexes by Electrospray Ionization Mass SpectrometryJournal of the American Chemical Society, 1994
- Mass spectrometric studies on noncovalent dimers of leucine zipper peptidesJournal of the American Chemical Society, 1993
- Detection of oligonucleotide duplex forms by ion-spray mass spectrometryTetrahedron Letters, 1993
- Principles and practice of electrospray ionization—mass spectrometry for large polypeptides and proteinsMass Spectrometry Reviews, 1991
- Electrospray ionization–principles and practiceMass Spectrometry Reviews, 1990
- Interpreting mass spectra of multiply charged ionsAnalytical Chemistry, 1989
- The Antibiotics of the Pluramycin Group (4 H-Anthra [1,2-b] pyran Antibiotics)1986