Silver Nanoparticle Conjugated Star PCL-b-AMPs Copolymer as Nanocomposite Exhibits Efficient Antibacterial Properties

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Abstract
The traditional antibiotics have specific intracellular targets and disinfect in chemical ways, and the drug-resistance induced by the antibiotics has grown into an emerging threat. It is an urgent to call for novel strategies and antibacterial materials to control this situation. Herein, we report a class of silver-decorated nanocomposite [email protected] as potent nano-antibiotic, constructed by ring-opening polymerization of the monomers ε-caprolactone, Z-Lys-N-carboxyanhydrides (NCAs) and Phe-NCAs, then decorated with AgNPs, and characterized by SEM, TEM and DLS. The biological assays revealed that the nanocomposite possessed strong antibacterial efficacy against both Gram-positive and Gram-negative bacteria including clinic isolated bacteria MRSA, VRE, P. aeruginosa, and K. pneumonia, exhibiting a MIC value range in 2-8 μg/mL. Importantly, the S. aureus and P. aeruginosa treated with the nanocomposite didn’t show drug-resistance even after 21th passages. Also, in vivo anti-infective assays showed that the nanocomposite was able to effectively kill bacteria in the infected viscera of mice. The study of the sterilization mechanism showed that the nanocomposite exhibited a multimodal antimicrobial mechanism, including irreversibly damaged the membrane structure, made the leakage of intracellular ions and subsequently induced generation of the reactive oxygen species (ROS), ultimately sterilized bacteria. The nanocomposite exhibit effective broad-spectrum antibacterial property and show low toxicity to the mammalian cells /animal. Overall, the [email protected] gained in this work show great potential as a highly promising antibacterial material for biomedical applications including drug-resistant bacterial infection.
Funding Information
  • National Natural Science Foundation of China (21572179)
  • Shaanxi Province (2019KW-068)

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