Leptographium bhutanense sp. nov., associated with the root collar weevil Hylobitelus chenkupdorjii on Pinus wallichiana in Bhutan
Open Access
- 1 December 2008
- journal article
- Published by Westerdijk Fungal Biodiversity Institute in Persoonia - Molecular Phylogeny and Evolution of Fungi
- Vol. 21 (1) , 1-8
Abstract
Leptographium spp. are commonly associated with bark beetles and weevils (Coleoptera: Curculionidae), and some are important tree pathogens. In a recent survey of diseases and insect pests of conifer trees in Bhutan, the root collar weevil, Hylobitelus chenkupdorjii was found girdling young Himalayan blue pine (Pinus wallichiana) trees in Central Bhutan. Intensive wood staining and a Leptographium sp. were associated with damage by this insect. The fungus was also isolated from individuals of H. chenkupdorjii. It was tentatively identified based on morphology and then compared with other Leptographium spp. using DNA sequences for three gene regions. Morphological characteristics showed that the Leptographium sp. from H. chenkupdorjii is similar to, but distinct from L. procerum and L. profanum. DNA sequence comparisons revealed that the isolates from Bhutan resided in a distinct well-supported clade and confirmed that they represent an undescribed taxon for which the name Leptographium bhutanense sp. nov. is provided.Keywords
This publication has 12 references indexed in Scilit:
- Multi-gene phylogenies define Ceratocystiopsis and Grosmannia distinct from OphiostomaStudies in Mycology, 2006
- Leptographium profanumsp.nov., a new species from hardwood roots in North AmericaCanadian Journal of Botany, 2006
- Leptographium wingfieldii introduced into North America and found associated with exotic Tomicus piniperda and native bark beetlesMycological Research, 2004
- Three new species of Leptographium from pineMycological Research, 2001
- Integrating Ambiguously Aligned Regions of DNA Sequences in Phylogenetic Analyses Without Violating Positional HomologySystematic Biology, 2000
- The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis toolsNucleic Acids Research, 1997
- Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetesApplied and Environmental Microbiology, 1995
- Ophiostoma himal-ulmi sp. nov., a new species of Dutch elm disease fungus endemic to the HimalayasMycological Research, 1995
- Distribution of Hylobius pales and Pissodes nemorensis (Coleoptera: Curculionidae) within Christmas Tree Plantations with Procerum Root DiseaseEnvironmental Entomology, 1992
- Reclassification of Verticicladiella based on conidial developmentTransactions of the British Mycological Society, 1985