Construction of a site-specific, deletion-frameshift mutation in an essential gene of bacteriophage ΦX174
Authors:
- M. Zafri HumayunMHM. Zafri Humayun
- Robert W. ChambersRCRobert W. Chambers
- 1 April 1979
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 278 (5704) , 524-529
Abstract
By in vitro methods, 80 nucleotides from a preselected site in the coding sequence of gene G of bacteriophage .PHI.X174 were deleted. This mutant (G.del./FS ZH1) also carries a -2 frameshift. The mutation is lethal, but mutant virus can be stably maintained on host strains bearing plasmids carrying .PHI. gene G.Keywords
This publication has 17 references indexed in Scilit:
- The nucleotide sequence of bacteriophage φX174Journal of Molecular Biology, 1978
- Construction and characterization of an Escherichia coli plasmid bearing a functional gene G of bacteriophage phiX174.Proceedings of the National Academy of Sciences, 1978
- Completed DNA sequences and organization of repressor-binding sites in the operators of phage lambdaJournal of Molecular Biology, 1977
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- Nucleotide sequence of bacteriophage φX174 DNANature, 1977
- Transcription of bacteriophage φX174 in vitro: Selective initiation with oligonucleotidesJournal of Molecular Biology, 1976
- A simple method of preparing large amounts of ΦX174 RF I supercoiled DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Fractionation of X174 Specific Messenger RNACold Spring Harbor Symposia on Quantitative Biology, 1970
- Structure of the replicative form of bacteriophage φX174Journal of Molecular Biology, 1968
- Infection of Protoplasts of Escherichia coli by Subviral Particles of Bacteriophage φX174Journal of Molecular Biology, 1960