Simultaneous multiplane imaging with reverberation two-photon microscopy
- 10 February 2020
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 17 (3) , 283-286
Abstract
Multiphoton microscopy has gained enormous popularity because of its unique capacity to provide high-resolution images from deep within scattering tissue. Here, we demonstrate video-rate multiplane imaging with two-photon microscopy by performing near-instantaneous axial scanning while maintaining three-dimensional micrometer-scale resolution. Our technique, termed reverberation microscopy, enables the monitoring of neuronal populations over large depth ranges and can be implemented as a simple add-on to a conventional design.Keywords
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Funding Information
- National Science Foundation (EEC-0812056, EEC-0812056, EEC-0812056)
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