The novel regulatory ncRNA, NfiS, optimizes nitrogen fixation via base pairing with the nitrogenase gene nifK mRNA in Pseudomonas stutzeri A1501
- 12 July 2016
- journal article
- research article
- Published by National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 113 (30) , E4348-E4356
Abstract
Significance: The biological nitrogen fixation process responsible for the reduction of atmospheric nitrogen to ammonia represents the primary source of nitrogen supporting extant life. We have identified a novel noncoding RNA (ncRNA) in the Pseudomonas stutzeri core genome, called NfiS, that is involved in the stress response and controls the expression of genes located in a genomic nitrogen-fixing ( nif ) island. NfiS was found to optimize nitrogen fixation by the direct posttranscriptional regulation of nitrogenase gene nifK mRNA. The acquisition of the nif island and the recruitment of NfiS by nifK mRNA are evolutionary events that seem to contribute to fine-tuned regulation of nitrogenase activity in P. stutzeri . This study provides a new regulatory pathway, mediated by an ncRNA for optimal nitrogen fixation, that may operate in other diazotrophs.Keywords
Funding Information
- Ministry of Science and Technology of the People's Republic of China (2015CB755700)
- National Natural Science Foundation of China (31230004)
- National Natural Science Foundation of China (31470205)
- National Natural Science Foundation of China (31470174)
- Ministry of Science and Technology of the People's Republic of China (2012AA02A703)
This publication has 60 references indexed in Scilit:
- Dual function of the McaS small RNA in controlling biofilm formationGenes & Development, 2013
- Targeting of csgD by the small regulatory RNA RprA links stationary phase, biofilm formation and cell envelope stress in Escherichia coliMolecular Microbiology, 2012
- Evolutionary Dynamics of Small RNAs in 27 Escherichia coli and Shigella GenomesGenome Biology and Evolution, 2012
- Regulation by Small RNAs in Bacteria: Expanding FrontiersMolecular Cell, 2011
- Molecular Interaction Studies Using Microscale ThermophoresisASSAY and Drug Development Technologies, 2011
- Positive regulation by small RNAs and the role of HfqProceedings of the National Academy of Sciences, 2010
- Small RNA as global regulator of carbon catabolite repression in Pseudomonas aeruginosaProceedings of the National Academy of Sciences, 2009
- Nitrogen fixation island and rhizosphere competence traits in the genome of root-associated Pseudomonas stutzeri A1501Proceedings of the National Academy of Sciences, 2008
- An internal antisense RNA regulates expression of the photosynthesis gene isiAProceedings of the National Academy of Sciences, 2006
- Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicumGene, 1994