Supplementary Methods and Figures S1 to S7 from CSF1/CSF1R Signaling Inhibitor Pexidartinib (PLX3397) Reprograms Tumor-Associated Macrophages and Stimulates T-cell Infiltration in the Sarcoma Microenvironment

Authors:
Open Access
Publisher website
Google Scholar
Abstract
Supplementary data file shows supplementary methods and figures S1 to S7. Supplementary Figure S1 shows the establishment of the in vitro sarcoma TAM model. Supplementary Figure S2 shows the effect of anti-CSF-1 and anti-CSF-1R mAb and pazopanib on in vitro sarcoma TAMs. Supplementary Figure S3 show spontaneous lung metastasis in LM8 orthotopic osteosarcoma mouse model. Supplementary Figure S4 shows CSF-1R inhibition by PLX3397 blocks LM8-luc tumor growth and lung metastasis in orthotopic osteosarcoma mouse model. Supplementary Figure S5 shows toxicity assessment by body weight and blood chemistry per treatment group at the end of therapeutic experiments (day 22). Supplementary Figure S6 show histopathological examination of orthotopic osteosarcoma mouse models. Supplementary Figure S7 shows alteration of the TME composition in PLX3397-treated mice after CSF-1R inhibition