High resolution visual mapping of stretched DNA by fluorescent hybridization
Authors:
- Bradford WindleBWBradford Windle
- 1 September 1993
- journal article
- Published by Springer Nature in Nature Genetics
- Vol. 5 (1) , 17-21
Abstract
We describe a method for stretching DNA, which, when combined with fluorescent hybridization procedures, forms a new mapping technology that produces a high resolution, vivid, multi-colour image and map. Restriction fragments and cosmid probes were successfully mapped by this procedure with validation by standard restriction mapping. A long range map of a > 200 kilobase region containing five copies of the amplified dihydrofolate reductase gene was easily generated within two days. This DNA mapping procedure offers a significant and rapid alternative to a variety of standard mapping procedures.Keywords
This publication has 16 references indexed in Scilit:
- Extending the capabilities of interphase chromatin mappingNature Genetics, 1992
- High-resolution in situ hybridization using DNA halo preparationsHuman Molecular Genetics, 1992
- Estimating Genomic Distance from DNA Sequence Location in Cell Nuclei by a Random Walk ModelScience, 1992
- A central role for chromosome breakage in gene amplification, deletion formation, and amplicon integration.Genes & Development, 1991
- Radiation Hybrid Mapping: A Somatic Cell Genetic Method for Constructing High-Resolution Maps of Mammalian ChromosomesScience, 1990
- Chromosomal Region of the Cystic Fibrosis Gene in Yeast Artificial Chromosomes: A Model for Human Genome MappingScience, 1990
- Interphase and Metaphase Resolution of Different Distances Within the Human Dystrophin GeneScience, 1990
- High-Resolution Mapping of Human Chromosome 11 by in Situ Hybridization with Cosmid ClonesScience, 1990
- The proximity of DNA sequences in interphase cell nuclei is correlated to genomic distance and permits ordering of cosmids spanning 250 kilobase pairsGenomics, 1989
- Effect of gamma rays at the dihydrofolate reductase locus: Deletions and inversionsSomatic Cell and Molecular Genetics, 1986