HUMAN ANTI-CENTROMERE SERA RECOGNIZE A 19.5 KD NON-HISTONE CHROMOSOMAL PROTEIN FROM HELA-CELLS

Authors:
  • HGHH GULDNER
    HH GULDNER
  • HLHJ LAKOMEK
    HJ LAKOMEK
  • FBFA BAUTZ
    FA BAUTZ
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  • 1 January 1984
    • journal article
    • research article
Abstract
Autoimmune sera from 18 scleroderma patients gave a centromere positive immunofluorescence response on formaldehyde fixed HeLa [human cervical carcinoma] cells and on chromosome spreads. Immunoblotting experiments with a protein fraction enriched in HeLa chromosomal proteins revealed that the antigenic target common to all 18 sera is a polypeptide of 19.5 kD [kilodaltons]. This polypeptide, which is not one of the core histones, is not soluble under conditions which favor the release of nuclear ribonucleoprotein particles. Antigen specific purification of autoantibodies with subsequent immunofluorescence studies confirmed that the 19.5 kD antigen is restricted to cell cycle-dependent single or double spheres at the centromere of HeLa chromosomes. Two additional polypeptides of 23 kD and 25.5 kD immunoreactive with 5 of the 18 centromere positive sera are not located at the centromere region, suggesting that other autoantibody systems are present in these sera.

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