Activity, diversity and population size of ammonia-oxidising bacteria in oil-contaminated landfarming soil
Authors:
- Jukka KurolaJKJukka Kurola
- Mirja Salkinoja-SalonenMSMirja Salkinoja-Salonen
- Tuula AarnioTATuula Aarnio
- Jenni HultmanJHJenni Hultman
- Martin RomantschukMRMartin Romantschuk
- 1 September 2005
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 250 (1) , 33-38
Abstract
Chemolithotrophic ammonia-oxidising bacteria (AOB) present in oil-contaminated landfarming soil were studied over two growing seasons in 1999 and 2000. The number of AOB (4-9 x 10(5) cellsg(-1) of dry soil) determined with the quantitative polymerase chain reaction (real-time PCR) and the rate of potential ammonium oxidation (0.05-0.28 microg NO2(-)-N g(-1) of dry soil h(-1)) indicated the presence of stable AOB populations. Denaturing gradient gel electrophoresis (DGGE) profiling and sequence analysis of PCR-amplified AOB 16S rRNA genes showed dominance of Nitrosospira-like sequences in clusters 2 and 3. The present results from the chronically oil-contaminated landfarming soil support the suggested importance of Nitrosospira-like AOB in terrestrial environments.Keywords
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