Light-Controlled Anticancer Activity and Cellular Uptake of a Photoswitchable Cisplatin Analogue
Authors:
- Marta StolarekMSMarta Stolarek
- Kamil KaminskiKKKamil Kaminski
- Marta Kaczor-KamińskaMKMarta Kaczor-Kamińska
- Magdalena ObłozaMOMagdalena Obłoza
- Piotr BonarekPBPiotr Bonarek
- Anna CzajaACAnna Czaja
- Magdalena DattaMDMagdalena Datta
- Mateusz BrelaMBMateusz Brela
- Janusz RakJanusz Rak
- Maria NowakowskaMNMaria Nowakowska
Open Access
- 24 October 2024
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Medicinal Chemistry
- Vol. 67 (21) , 19103-19120
Abstract
A photoactive analogue of cisplatin was synthesized with two arylazopyrazole ligands, able to undergo trans-cis/cis-trans photoisomerizations. The cis photoisomer showed a dark half-life of 9 days. The cytotoxicities of both photoisomers of the complex were determined in several cancer and normal cell lines and compared to that of cisplatin. The trans photoisomer of the complex was much more cytotoxic than both the cis photoisomer and cisplatin, and was more toxic for cancer (4T1) than for normal (NMuMG) murine breast cells. 4T1 cell death occurred through necrosis. Photoisomerization of the trans and cis photoisomers internalized by the 4T1 cells increased and decreased their viability, respectively. The cellular uptake of the trans photoisomer was stronger than that of both the cis photoisomer and cisplatin. Both photoisomers interacted with DNA faster than cisplatin. The trans photoisomer was bound stronger by bovine serum albumin and induced a greater decrease in cellular glutathione levels than the cis photoisomer.Keywords
Funding Information
- Narodowe Centrum Nauki (UMO-2021/41/B/NZ7/02484)
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