Echinococcus granulosusstrain differentiation based on sequence heterogeneity in mitochondrial genes of cytochromecoxidase-1 and NADH dehydrogenase-1

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Abstract
Genetic analyses ofEchinococcus granulosusisolates from different intermediate host species have demonstrated substantial levels of variation for some genotype (strain) clusters. To determine the range of genetic variability within and between genotypes we amplified and cloned partialcox1andnadh1genes from 16 isolates ofE. granulosusfrom 4 continents. Furthermore, we sequenced different clones from a PCR product to analyse the intra-individual genetic variance. The findings showed a moderate degree of variance within single isolates and a significant degree of variance between the cluster of genotypes G1–G3 (sheep, Tasmanian sheep and buffalo strain), genotypes G4 (horse strain) and G5 (cattle strain) and the cluster of the genotypes G6 (camel strain) and G7 (pig strain). The variance of up to 2·2% within genotypes was relatively low compared with that of 4·3–15·7% among genotypes. The present results indicate that a re-examination of the classification of 5 genotypes ofEchinococcusis warranted. Hence, our data highly support a re-evaluation of the taxonomy of the clades G1–G3, G4, G5, G6/7 and G8 (cervid strain) within the genusEchinococcus.

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