Reestablishing an Intragastric Ethanol Self‐infusion Model in Rats

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Abstract
Background: There is a scarcity of behavioral models that will reliably produce ethanol intakes in rodents at levels that induce or maintain dependence. The present experiments were designed to reestablish a model that uses passive intragastric (IG) infusion of ethanol to induce tolerance/dependence/withdrawal before allowing rats to self‐infuse ethanol intragastrically.Methods: Sprague–Dawley rats were surgically implanted with IG catheters and allowed to recover. During the passive infusion phase (3–6 days), rats in the experimental group were passively infused with 10% (v/v) ethanol (3.3–12.2 g/kg/d). Rats in the control group were not infused. During the self‐infusion phase (5–6 days), all rats had access to 2 flavored solutions. Licks on 1 solution were paired with ethanol infusions (20%, v/v) whereas licks on the other solution were unpaired. Experiments differed in the specific passive infusion parameters and in the ethanol intake limit during self‐infusion.Results: Rats in the experimental groups self‐infused more ethanol per day (means of 4–7 g/kg/d) than did rats in the control group (means of 0–2.6 g/kg/d). Across all 3 studies, individual total daily intakes exceeded 5 g/kg on 35% of the self‐infusion days in ethanol‐preexposed rats compared with 1.5 g/kg) compared with control rats (Conclusions: Relatively short periods of passive IG infusion of ethanol induced levels of ethanol self‐infusion in genetically heterogeneous rats that were comparable with drinking intakes previously reported in rats selectively bred for ethanol intake/preference. Although the induction of dependence/withdrawal may have played a role in this outcome, an alternative interpretation is that experimental rats self‐infused more ethanol because passive exposure produced tolerance to aversive pharmacological effects that would otherwise limit intake of the paired flavor because of development of conditioned taste aversion. The current findings provide a strong basis for future work designed to identify parametric determinants of this form of self‐administration, its sensitivity to genetic influences, and its neurobiological substrates.

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