Amount of nickel applied with a standard patch test

Authors:
Publisher website
Google Scholar
Abstract
The concentration of nickel sulphate was determined in 9‐16 mg test samples from commercially available patch test antigens. With two different manufacturers of 2.5% nickel sulphate in pet, the concentration range of 25 samples was 0.7 and 1.1% (±2 SD) respectively, and with one manufacturer of 5.0% nickel sulphate in pet, the range was ±0.5%. In a normal patch test, the volume of patch test material applied with the Finn chamber technique should be 12–18μl (9–16 mg), but has been found to range between 9 and 50 μl. Thus the amount of nickel applied in standard patch tests varies within at least 6‐fold limits.