Differential labeling of the cell surface of single ciliary ganglion neurons in vitro.
Authors:
- N K WessellsNWN K Wessells
- R P NuttallRNR P Nuttall
- J T WrennJWJ T Wrenn
- S JohnsonSJS Johnson
- 1 November 1976
- journal article
- research article
- Published by National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 73 (11) , 4100-4104
Abstract
Cationic ferritin bound in a time and concentration dependent manner to all surfaces of [chick embryo] ciliary ganglion neurons in culture except mounds and veils. In chase experiments, bound ferritin cleared from the cell surfaces and formed larger and larger patches, even at low temperatures. Binding of cationic ferritin was inhibited by poly-L-lysine, potentiated by poly-L-glutamate and not affected by neuraminidase (acylneuraminyl hydrolase, EC 3.2.1.18), hyaluronidase (hyaluronoglucosidase, hyaluronate 4-glycanhydrolase, EC 3.2.1.35) or chondroitin ABC lyase (EC 4.2.2.4).Keywords
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