Characterization of the Single‐Strand‐Specific Nuclease S1 Activity on Double‐Stranded Supercoiled Polyoma DNA
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Open Access
- 1 April 1974
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 43 (3) , 591-600
Abstract
The DNA of polyoma virus consists of double‐stranded supercoiled circular molecules. The single‐strand‐specific nuclease S1 converts these molecules to unit length rods. The conversion occurs in a two‐step reaction, with nuclease S1 cutting first one of the two strands and then the intact strand opposite to this nick. Cleavage of intact polyoma DNA by nuclease S1 is a consequence of supercoiling. DNA molecules containing random single‐strand breaks introduced by physical or enzymatic means are also cut opposite to nicks.Keywords
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