Measurement of Protein−Ligand Binding Constants from Reaction-Diffusion Concentration Profiles
Authors:
- 5 October 2010
- journal article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 82 (21) , 8780-8784
Abstract
Protein-ligand dissociation constants, K(d), are determined precisely and down to the picomolar range from reaction-diffusion (RD) concentration profiles created by proteins diffusing through hydrogels functionalized with protein ligands. The RD process effectively amplifies the molecular-scale binding events into macroscopic patterns visible to the naked eye. The method is applicable to various protein-ligand pairs and does not require any prior knowledge about the protein structure.Keywords
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