Figure 2 from Characterization of Mitochondrial Double-Stranded RNA Levels in Non–Small Cell Lung Carcinoma

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Abstract
Enrichment of mtdsRNAs is captured through dsRNA-seq in selected NSCLC cell lines. A, Workflow of RIP-seq. B, Quantification of unique reads detected by RIP-seq. Reads from the heavy (red) and light (blue) strands were mapped to the mitochondrial genome ChrMT. See Supplementary Fig. S1 for an enlarged view of the sequencing data. C and D, Statistical analysis of successfully aligned reads per genomic feature (gene, CDS, rRNA, exon, and D-loop, with either tRNA included or excluded). Bar represents average. Error bars represent ± SEM. P values are represented as is. One-way ANOVA was completed. Each dot represents an independent experiment (n = 3). E, Mitochondrial genome mapped with primers used for long-read sequencing. Primers can be found in Supplementary Table S1. Green: ND5 Fwd → RNR2 Rev. Blue: RNR2 Fwd → Cox3 Rev. Orange: Cox3 Fwd → ND5 Rev. F, Gel showing the bands generated by PCR across three different cell lines used for ADAR sequencing. Representative of three independent experiments (n = 3).