DNA Isolation from Mammalian Samples
Authors:
Open Access
- 1 April 2013
- journal article
- unit
- Published by Wiley in Current Protocols in Molecular Biology
- Vol. 102 (1) , 2.14.1-2.14.13
Abstract
DNA isolation is a fundamental initial step for molecular genetic studies. Two quite different methodologies are described in this unit: silica spin column and phenol/chloroform extraction. Currently, the most commonly used technique is the silica‐based spin column, although phenol/chloroform extraction is still widely used. This unit also presents basic procedures for extraction of DNA from both fresh tissues and formalin‐fixed, paraffin‐embedded (FFPE) tissue samples. Curr. Protoc. Mol. Biol. 102:2.14.1–2.14.13. © 2013 by John Wiley & Sons, Inc.Keywords
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