Carbapenemase-producing Klebsiella pneumoniae in the Czech Republic in 2011
Authors:
- J HrabákJHJ Hrabák
- C C PapagiannitsisC C Papagiannitsis
- H ZemlickovaHZH Zemlickova
- Czech Participants Of European Antimicrobial Resistance Surveillance NetworkCNCzech Participants Of European Antimicrobial Resistance Surveillance Network
Open Access
- 7 November 2013
- journal article
- Published by European Centre for Disease Control and Prevention (ECDC) in Eurosurveillance
- Vol. 18 (45) , 20626
Abstract
Carbapenemase-producing Enterobacteriaceae and Pseudomonas spp. are increasingly reported in many countries all over the world. Due to the resistance of those bacteria to almost all antibiotics (e.g. beta-lactams, aminoglycosides, fluoroquinolones), treatment options are seriously limited. In the Czech Republic, the incidence of carbapenemase-producing Enterobacteriaceae seems to be low, restricted to only three cases detected between 2009 and 2010. Here, we describe molecular typing of 15 carbapenemase-producing Klebsiella pneumoniae isolates identified in the Czech Republic during 2011. Five VIM-1-producing isolates belonging to sequence type (ST) 11 and one VIM-4-producing isolate of ST1029 have been detected. blaVIM-1 and blaVIM-4 as a part of class 1 integrons were chromosomally located or carried by a plasmid belonging to A/C replicon type (blaVIM-4). KPC-3-producing isolates of ST512, recovered from six patients, caused an outbreak. Three more isolates producing KPC-2 enzyme belonged to ST258. Both blaKPC genes were part of the Tn4401a transposon carried on plasmids of the pKpQIL type. The isolates were resistant to all antibiotics tested except colistin and/or gentamicin. Four of these 15 strains were recovered from patients repatriated to the Czech Republic from Greece and Italy. This is the first report of outbreaks caused by carbapenemase-producing Enterobacteriaceae in the Czech Republic.Keywords
This publication has 28 references indexed in Scilit:
- Detection of NDM-1, VIM-1, KPC, OXA-48, and OXA-162 Carbapenemases by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass SpectrometryJournal of Clinical Microbiology, 2012
- Rapid evolution and spread of carbapenemases among Enterobacteriaceae in EuropeClinical Microbiology and Infection, 2012
- Interventional strategies and current clinical experience with carbapenemase-producing Gram-negative bacteriaClinical Microbiology and Infection, 2012
- Global Spread of Carbapenemase-producingEnterobacteriaceaeEmerging Infectious Diseases, 2011
- Carbapenemase Activity Detection by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass SpectrometryJournal of Clinical Microbiology, 2011
- KPC-2-producing Klebsiella pneumoniae isolated from a Czech patient previously hospitalized in Greece and in vivo selection of colistin resistanceFolia Microbiologica, 2011
- A sensitive and specific phenotypic assay for detection of metallo-β-lactamases and KPC in Klebsiella pneumoniae with the use of meropenem disks supplemented with aminophenylboronic acid, dipicolinic acid and cloxacillinClinical Microbiology and Infection, 2011
- Regional Spread of Pseudomonas aeruginosa ST357 Producing IMP-7 Metallo-β-Lactamase in Central EuropeJournal of Clinical Microbiology, 2011
- Carbapenem-non-susceptible Enterobacteriaceae in Europe: conclusions from a meeting of national expertsEurosurveillance, 2010
- Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by broth dilutionClinical Microbiology and Infection, 2003