In Situ Fixation of the Neonatal Brain and Spinal Cord

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Abstract
A delay in the autopsy can result in significant tissue autolysis, especially in the central nervous system. We have developed a rapid technique of in situ fixation that preserves central nervous system tissues until the formal autopsy can be performed. Through the lateral margin of the anterior fontanelle, Zamboni's solution is injected percutaneously into the lateral venticles and allowed to exit via an intrathecal spinal needle. The choice of fixative allows a wide array of postmorten studies to be done.

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