Isolation and Characterization of Flavonol Glycosides from Leaves Extract of Lupinus arboreus Sims

Authors:
  • SOSylvester C. Ohadoma
    Sylvester C. Ohadoma
  • PAPeter A. Akah
    Peter A. Akah
  • COChris E. Okolo
    Chris E. Okolo
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Abstract
We planned to isolate the active constituents from methanol extract of Lupinus arboreus leaves. The crude methanol extract (CME) was fractionated into hexane, ethyl acetate and methanol fractions. The fractions were isolated from silica gel column chromatography, which were eluted with gradient mixtures. The isolated fractions were characterized by using flavonoid screening, IR, UV and NMR spectral analyses. The result of the phytochemical analysis and chromatography of the ethyl acetate fraction over silica gel lead to the isolation of a flavonol glycosides, and identified as tetrahydroxyflavone-3α-rhamnoside. Bioactivity-guided result indicated tetrahydroxyflavone-3α-rhamnoside and imparts anti-inflammatory compound in this plant.