Quantitative Determination of Aucubin in SevenPlantagoSpecies Using HPLC, HPTLC, and LC-ESI-MS Methods
- 12 October 2010
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 43 (16) , 2487-2495
Abstract
A reversed-phase high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of aucubin in Plantago lanceolata. The analyses were carried out on Zorbax SB-C18 column with an aqueous phosphoric acid and acetonitrile gradient. The correlation coefficient of calibration curve showed good linearity (r > 0.9995), with average recoveries between 96.7 and 104.5%. The developed method was applied for quantification in P. atrata, P. bellardii, P. coronopus, P. holosteum, P. reniformis, and P. schwarzenbergiana. The aucubin content in plant extracts was compared by HPLC, HPTLC, and LC-ESI-MS techniques and no significant differences between the conducted methods were observed.Keywords
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