Membrane-associated DNA Transport Machines
Authors:
Open Access
- 23 June 2010
- journal article
- review article
- Published by Cold Spring Harbor Laboratory in Cold Spring Harbor Perspectives in Biology
- Vol. 2 (7) , a000406
Abstract
DNA pumps play important roles in bacteria during cell division and during the transfer of genetic material by conjugation and transformation. The FtsK/SpoIIIE proteins carry out the translocation of double-stranded DNA to ensure complete chromosome segregation during cell division. In contrast, the complex molecular machines that mediate conjugation and genetic transformation drive the transport of single stranded DNA. The transformation machine also processes this internalized DNA and mediates its recombination with the resident chromosome during and after uptake, whereas the conjugation apparatus processes DNA before transfer. This article reviews these three types of DNA pumps, with attention to what is understood of their molecular mechanisms, their energetics and their cellular localizations.Keywords
This publication has 128 references indexed in Scilit:
- Pili in Gram-positive bacteria: assembly, involvement in colonization and biofilm developmentTrends in Microbiology, 2008
- DprA/Smf protein localizes at the DNA uptake machinery in competent Bacillus subtilis cellsBMC Microbiology, 2007
- Cell‐specific SpoIIIE assembly and DNA translocation polarity are dictated by chromosome orientationMolecular Microbiology, 2007
- The Unconventional Xer Recombination Machinery of Streptococci/LactococciPLOS Genetics, 2007
- The outer membrane secretin PilQ from Neisseria meningitidis binds DNAMicrobiology, 2007
- The tyrosine kinase McsB is a regulated adaptor protein for ClpCPThe EMBO Journal, 2007
- Role of an FtsK-Like Protein in Genetic Stability inStreptomyces coelicolorA3(2)Journal of Bacteriology, 2007
- NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion systemProceedings of the National Academy of Sciences, 2007
- The FtsK γ domain directs oriented DNA translocation by interacting with KOPSNature Structural & Molecular Biology, 2006
- Common components in the assembly of type 4 fimbriae, DNA transfer systems, filamentous phage and protein‐secretion apparatus: a general system for the formation of surface‐associated protein complexesMolecular Microbiology, 1993