Dual-Functionalized Apatite Nanocomposites with Enhanced Cytocompatibility and Osteogenesis for Periodontal Bone Regeneration

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Abstract
The development of biomimetic bone graft materials for periodontal tissue engineering is a field of topical interest. In this study, we designed a dual-functionalized apatite nanocomposite, which could integrate multiple molecular cues for manipulating the fate of periodontal ligament stem cells (PDLSCs). Briefly, inspired by mussels, a biomimetic nano-hydroxyapatite was fabricated using polydopamine structure as template (named as tHA), and then surface modified with bone forming peptide-1 (BFP-1) and vascular endothelial growth factor (VEGF)-mimicking peptide (QK) via a single step of catechol chemistry. Our study showed that the biofunctions of tethered peptides were not compromised on the surface of apatite nanoparticles. Due to the synergistic effect of BFP-1 and QK peptides, the dual-functionalized apatite nanocomposite showed improved cytocompatibility compared to controls. Moreover, it can boost the proliferation and osteogenic differentiation of PDLSCs, indicating excellent bioactivity of tHA-BFP/QK nanoparticles on cell fate decision. More importantly, animal experiments showed that dual-functionalized apatite nanocomposite could dramatically promote the regeneration of periodontal bone regeneration. It is concluded that our work provides an instructive insight into the design of biomimetic apatite nanocomposite, which hold a great potential for application in periodontal bone repair.KEYWORDS: apatite, peptide, dental, periodontal tissue engineering
Funding Information
  • Chongqing Municipal Education Commission (KJQN201900427)
  • China Postdoctoral Science Foundation (2019M663893XB)
  • Chongqing Postdoctoral Science Foundation
  • National Natural Science Foundation of China (31700850, 81570981)
  • Health Commission of Sichuan Province (17DZXM0200)
  • Program for Innovation Team Building at Institutions of Higher Education in Chongqing in 2013
  • Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education

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