The immunohistochemical localization of lysozyme in plaice (Pleuronectes platessa L.) tissues
Authors:
- 1 October 1976
- journal article
- research article
- Published by Wiley in Journal of Fish Biology
- Vol. 9 (4) , 329-334
Abstract
The location of lysozyme in fresh plaice (Pleuronectes platessa L.) tissues was studied using an immunofluorescent technique. An antiserum to a homogeneous sample of plaice serum lysozyme was prepared in rabbits and was shown to be monospecific by immunodiffusion and inhibition studies. This preparation, used in conjunction with fluorescent labelled sheep anti‐rabbit immunoglobulin, demonstrated lysozyme in plaice neutrophils, peritoneal macrophages, gill cartilage and epithelium, spleen, kidney, skin and mucus. The distribution of lysozyme in the skin and secreted mucus was affected by the method of killing the fish.Keywords
This publication has 13 references indexed in Scilit:
- The lysozyme of the plaice Pleuronectes platessa L.Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1976
- Complex formation between lysozyme and cartilage proteoglycansBiochimica et Biophysica Acta (BBA) - Protein Structure, 1974
- Lysozyme of Cartilage and Other Connective Tissues (A Speculative Review)1974
- Lysozyme activity in the plaice (Pleuronectes platessa L.)Cellular and Molecular Life Sciences, 1973
- Studies on the skin of plaice (Pleuronectes platessa L.)Journal of Fish Biology, 1972
- The cytochemical localization of lysozyme in Paneth cell granulesJournal of Molecular Histology, 1971
- Multiple genes for lysozyme in birdsArchives of Biochemistry and Biophysics, 1970
- HISTOCHEMICAL DISTRIBUTION OF LYSOZYME ACTIVITY IN ORGANS OF NORMAL MICE AND RADIATION CHIMERASJournal of Histochemistry & Cytochemistry, 1964
- Disk Electrophoresis of Basic Proteins and Peptides on Polyacrylamide GelsNature, 1962
- Occurrence of Heparin in the Blood of Fish and its Prevention of the Clotting of Fish PlasmaNature, 1961