Microscopic hyperspectral imaging used as a bio-optical taxonomic tool for micro- and macroalgae

In the presented study a hyperspectral imager ($400–700\mathrm{nm}$) mounted on a stereo-microscope was used to separate differences in in vivo optical signatures identifying different pigment groups of bloom-forming phytoplankton and macroalgae by comparing spectral absorption, transmittance, and reflectance from $400–700\mathrm{nm}$. The results show that the hyperspectral imager could be used to detect spectral characteristics on the $\mathrm{\mu m}$ level to calibrate, validate, identify, and separate objects with differences in color (optical fingerprinting). This information can be used for pigment group specific taxonomy (bio-optical taxonomy), eco-physiological information (e.g., health status), monitoring, and mapping applications.