Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2
Authors:
- Clinton R. PadenCPClinton R. Paden
- Ying TaoYTYing Tao
- Krista QueenKQKrista Queen
- Jing ZhangJZJing Zhang
- Yan LiYLYan Li
- Anna UeharaAUAnna Uehara
- Suxiang TongSTSuxiang Tong
- 1 October 2020
- journal article
- research article
- Published by Centers for Disease Control and Prevention (CDC) in Emerging Infectious Diseases
- Vol. 26 (10) , 2401-2405
Abstract
We describe validated protocols for generating high-quality, full-length severe acute respiratory syndrome coronavirus 2 genomes from primary samples. One protocol uses multiplex reverse transcription PCR, followed by MinION or MiSeq sequencing; the other uses singleplex, nested reverse transcription PCR and Sanger sequencing. These protocols enable sensitive virus sequencing in different laboratory environments.Keywords
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