Electrochemiluminescence Biosensor for Hyaluronidase Based on the Ru(bpy)32+ Doped SiO2 Nanoparticles Embedded in the Hydrogel Fabricated by Hyaluronic Acid and Polyethylenimine

Authors:
Publisher website
Google Scholar
Abstract
Hyaluronidase (HAase), a specific enzyme of hyaluronic acid (HA), has been reported as a potential tumor biomarker in recent years. Hence, developing some simple, rapid and sensitive methods for HAase assay is necessary. In this work, a simple and sensitive biosensor constructed by the reliable controlled release system and the mature electrochemiluminescence (ECL) analytical technique has been devised for the quantification of HAase with high efficiency and selectivity. tris (2,2'-bipyridyl) ruthenium (II) chloride hexahydrate doped SiO2 nanoparticles ([email protected]2 NPs), as ECL signal probes, were trapped in the hydrogel fabricated by HA and polyethylenimine evenly and steadily. When HAase existed, the hydrogel was decomposed by HAase, and the [email protected]2 NPs escaped from the hydrogel into the supernate. Then the ECL signal produced from the supernate can be detected and used to characterize HAase concentration. The result showed a good linear relationship between ECL intensity and HAase concentration ranged from 2 to 60 U/mL, and the limit of detection was 2 U/mL. The developed controlled release ECL biosensor has been used for HAase quantification in urine samples with satisfactory results.
Funding Information
  • STS Key Project of Fujian Province (2017T3007)
  • the Program for Changjiang Scholars and Innovative Research Team in University (No. IRT15R11)
  • National Sciences Foundation of China (21575025, 21575027, 21775026)
  • the cooperative project of production and study in University of Fujian Province (2018Y4007)
  • Sciences Foundation of Fujian Province (2018J01682, 2018J01685)

This publication has 43 references indexed in Scilit: