Pyrroloquinoline Quinine Inhibits RANKL-Mediated Expression of NFATc1 in Part via Suppression of c-Fos in Mouse Bone Marrow Cells and Inhibits Wear Particle-Induced Osteolysis in Mice
Authors:
Open Access
- 17 April 2013
- journal article
- retracted article
- Published by Public Library of Science (PLoS) in PLOS ONE
- Vol. 8 (4) , e61013
Abstract
The effects of pyrroloquinoline quinine (PQQ) on RANKL-induced osteoclast differentiation and on wear particle-induced osteolysis were examined in this study. PQQ inhibited RANKL-mediated osteoclast differentiation in bone marrow macrophages (BMMs) in a dose-dependent manner without any evidence of cytotoxicity. The mRNA expression of c-Fos, NFATc1, and TRAP in RANKL-treated BMMs was inhibited by PQQ treatment. Moreover, RANKL-induced c-Fos and NFATc1 protein expression was suppressed by PQQ. PQQ additionally inhibited the bone resorptive activity of differentiated osteoclasts. Further a UHMWPE-induced murine calvaria erosion model study was performed to assess the effects of PQQ on wear particle-induced osteolysis in vivo. Mice treated with PQQ demonstrated marked attenuation of bone erosion based on Micro-CT and histologic analysis of calvaria. These results collectively suggested that PQQ demonstrated inhibitory effects on osteoclast differentiation in vitro and may suppress wear particle-induced osteolysis in vivo, indicating that PQQ may therefore serve as a useful drug in the prevention of bone loss.Keywords
This publication has 38 references indexed in Scilit:
- Do Tissues From THA Revision of Highly Crosslinked UHMWPE Liners Contain Wear Debris and Associated Inflammation?Clinical Orthopaedics and Related Research, 2011
- Inhibition of osteoclast differentiation and bone resorption by rotenone, through down-regulation of RANKL-induced c-Fos and NFATc1 expressionBone, 2010
- Pyrroloquinoline Quinone Stimulates Mitochondrial Biogenesis through cAMP Response Element-binding Protein Phosphorylation and Increased PGC-1α ExpressionJournal of Biological Chemistry, 2010
- NF-κB functions in osteoclastsBiochemical and Biophysical Research Communications, 2009
- Differential effects of biologic versus bisphosphonate inhibition of wear debris‐induced osteolysis assessed by longitudinal micro‐CTJournal of Orthopaedic Research, 2008
- Nuclear Factor of Activated T Cells c1 Induces Osteoclast-associated Receptor Gene Expression during Tumor Necrosis Factor-related Activation-induced Cytokine-mediated OsteoclastogenesisJournal of Biological Chemistry, 2005
- Expression of inflammatory cytokines, RANKL and OPG induced by titanium, cobalt-chromium and polyethylene particlesBiomaterials, 2005
- Osteoclast differentiation and activationNature, 2003
- Pyrroloquinoline Quinone Acts with Flavin Reductase to Reduce Ferryl Myoglobin in Vitro and Protects Isolated Heart from Reoxygenation InjuryBiochemical and Biophysical Research Communications, 1993
- Quinone Derivatives Lower Blood and Liver Acetaldehyde but Not Ethanol Concentrations following Ethanol Loading to RatsPharmacology, 1988