Analysis of the Genetic Structure of Slovak Holstein Cattle Using Seven Candidate Genes Related to Milk Quality

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Abstract
Milk is an important component of human nutrition, and its composition and milk yield fundamentally affect the economy of dairy farms. Genetic variability is a fundamental premise for livestock breeding and is commonly used in the identification of individual animals and in selection to improve performance. The aims of this study were to propose a rapid detection method for genes affecting the nutritional value and technological properties of bovine milk (FADS1, FADS2, FASN, SCD, DGAT1, CSN2 and CSN3) and to analyze Slovak Holstein cattle to widen knowledge on their genetic structure for these candidate genes. Genotyping was performed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and artificially created restriction site-polymerase chain reaction (ACRS-PCR) methods. Heterozygosity is an important factor for estimating genetic variation in domestic animals and represents the genetic potential and ability to adapt to the natural environment. In this study, Holstein cattle showed high heterozygosity values for markers FADS1-07 and CSN2-H67P. In contrast, they showed high homozygosity values for markers FADS1-01, FADS2-23, FASN-16024, SCD-T878C, DGAT1-K232A and CSN3-D148A. These results suggest that genetic diversity has been reduced, which may be due to breeding effects.
Funding Information
  • Agentúra na Podporu Výskumu a Vývoja (APVV-17-0060, APVV-20-0161, 012SPU-4/2019, 313011W112)
  • Ministry of Education, Science, Research and Sport of the Slovak Republic (APVV-17-0060, APVV-20-0161, 012SPU-4/2019, 313011W112)
  • European Regional Development Fund (APVV-17-0060, APVV-20-0161, 012SPU-4/2019, 313011W112)

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