LRP‐1/TβR‐V mediates TGF‐β1‐induced growth inhibition in CHO cells
Authors:
- Jung San HuangJHJung San Huang
- 27 February 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 562 (1-3) , 71-78
Abstract
The type V transforming growth factor‐β (TGF‐β) receptor (TβR‐V) is hypothesized to be involved in cellular growth inhibition by TGF‐β1. Recently, TβR‐V was found to be identical to low density lipoprotein receptor‐related protein‐1 (LRP‐1). Here we demonstrate that TGF‐β1 inhibits growth of wild‐type CHO cells but not LRP‐1‐deficient mutant cells (CHO‐LRP‐1− cells). Stable transfection of CHO‐LRP‐1− cells with LRP‐1 cDNA restores the wild‐type morphology and the sensitivity to growth inhibition by TGF‐β1. In addition, overexpression of LRP‐1 minireceptors exerts a dominant negative effect and attenuates the growth inhibitory response to TGF‐β1 in wild‐type CHO cells. These results suggest that LRP‐1/TβR‐V is critical for TGF‐β1‐mediated growth inhibition in CHO cells.Keywords
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