5′‐Capping Structures of Artemia salina mRNA and the Translational Inhibition by Cap Analogs
Authors:
- 1 December 1976
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 71 (1) , 281-293
Abstract
The mRNA of the brine shrimp A. salina has 2 types of blocked methylated 5''-terminal structures (caps). About 75% of the mRNA molecules have the 5''-end structure m7G5''ppp5''-AmpGp and .apprx. 25% have the structure of m7G5''ppp5''GmpGp. The only other type of methylated residue found in Artemia mRNA is N6-methyladenosine located at internal positions along the mRNA chain. Translation of Artemia cyst or nauplius poly(A)-rich mRNA in wheat-germ extracts was inhibited by 7-methylguanosine 5''-monophosphate, a chemical analog of the cap, as well as by synthetic caps such as m7G5''ppp5''Gm. On the other hand, the elongation activity on endogenous mRNA in an Artemia cell-free system was not sensitive to 7-methylguanosine 5''-monophosphate.Keywords
This publication has 32 references indexed in Scilit:
- The Translation in vitro of mRNA from Developing Cysts of Artemia salinaEuropean Journal of Biochemistry, 1976
- Translational discrimination of ‘capped’ and ‘non‐capped’ mRNAS: inhibition by a series of chemical analogs of m7 GpppXFEBS Letters, 1976
- Cryptic form of mRNA in dormant artemia salina cystsBiochemical and Biophysical Research Communications, 1975
- Ribosome binding to reovirus mRNA in protein synthesis requires 5′ terminal 7-methylguanosineCell, 1975
- Modified nucleosides and bizarre 5′-termini in mouse myeloma mRNANature, 1975
- Methylated nucleotides block 5′ terminus of HeLa cell messenger RNACell, 1975
- The methylated constituents of L cell messenger RNA: Evidence for an unusual cluster at the 5′ terminusCell, 1975
- A blocked structure at the 5′ terminus of mRNA from cytoplasmic polyhedrosis virusNature, 1975
- Sequences containing methylated nucleotides at the 5′ termini of messenger RNAs: Possible implications for processingCell, 1974
- MITOCHONDRIAL BIOGENESIS DURING DIFFERENTIATION OF ARTEMIA SALINA CYSTSJournal of Cell Biology, 1973