Effect of the Host and Temperature on the Formation of Defective RNAs Associated with Broad bean mottle virus Infection

Authors:
  • SLSusana Llamas
    Susana Llamas
  • CSClaudio Sandoval
    Claudio Sandoval
  • MBMar Babin
    Mar Babin
  • JPJudy Pogany
    Judy Pogany
  • JBJozef J. Bujarski
    Jozef J. Bujarski
  • JRJavier Romero
    Javier Romero
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Abstract
Previously, we demonstrated that Broad bean mottle virus (BBMV), a member of the genus Bromovirus, could accumulate RNA 2-derived defective interfering (DI) RNAs during infection. In this work, we study how host and environmental factors affect the accumulation of DI RNAs. Serial passages of BBMV through selected plant species reveal that, with low-multiplicity inocula, some systemic hosts (Vicia faba, Nicotiana clevelandii, and N. tabacum cv. Samsum) support DI RNA accumulation after the first passage cycle but other hosts (Phaseolus vulgaris, Pisum sativum, and Glycine max) do not. However, several passages with the high-multiplicity inocula can generate DI RNAs in pea plants. Local lesion hosts (Chenopodium quinoa, C. amaranticolor, and C. murale) remain free of the DI RNA components. The size of the de novo-formed DI RNAs depends on the host and on environmental conditions. For instance, broad bean plants cultivated in a greenhouse or in a growth chamber at 20°C accumulated DI RNAs of 2.4 or ...

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