Creation of a transgenic mouse for hair‐cell gene targeting by using a modified bacterial artificial chromosome containing Prestin
Authors:
Open Access
- 1 July 2004
- journal article
- research article
- Published by Wiley in Developmental Dynamics
- Vol. 231 (1) , 199-203
Abstract
We made a transgenic mouse that expresses Cre recombinase activity in inner ear hair cells by using a modified bacterial artificial chromosome containing Prestin. Cre recombinase activity was restricted to inner and outer hair cells, a subset of vestibular hair cells, spiral and vestibular ganglia in the inner ear, and a subset of cells in the testis, epididymis, and ear bone. This mouse will be useful for hair‐cell–specific gene targeting. Developmental Dynamics 231:199–203, 2004.Keywords
This publication has 15 references indexed in Scilit:
- A gene expression atlas of the central nervous system based on bacterial artificial chromosomesNature, 2003
- Expression of prestin, a membrane motor protein, in the mammalian auditory and vestibular peripheryHearing Research, 2003
- Genomic characterization and expression of mouse prestin, the motor protein of outer hair cellsMammalian Genome, 2003
- Transgenic and gene targeting studies of hair cell function in mouse inner earJournal of Neurobiology, 2002
- Prestin is required for electromotility of the outer hair cell and for the cochlear amplifierNature, 2002
- Cochlear whole mount in situ hybridization: identification of longitudinal and radial gradientsBrain Research Protocols, 2002
- Targeting of cre to the Foxg1 (BF-1) Locus Mediates loxP Recombination in the Telencephalon and Other Developing Head StructuresDevelopmental Biology, 2000
- Prestin is the motor protein of cochlear outer hair cellsNature, 2000
- Visualization of α9 acetylcholine receptor expression in hair cells of transgenic mice containing a modified bacterial artificial chromosomeProceedings of the National Academy of Sciences, 1999
- Homologous recombination based modification in Esherichia coli and germline transmission in transgenic mice of a bacterial artificial chromsomeNature Biotechnology, 1997