Regulation of Ru(bpy)32+ Electrochemiluminescence Based on Distance-Dependent Electron Transfer of Ferrocene for Dual-Signal Readout Detection of Aflatoxin B1 with High Sensitivity

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Abstract
Ferrocene (Fc) is a common quencher of Ru(bpy)32+ luminescence. However, interactions between Fc and Ru(bpy)32+ can be extremely complicated. In this work, we reported the first use of Fc to regulate the electrochemiluminescence (ECL) of Ru(bpy)32+ by tuning the length of the DNA sequence between Fc and the luminophore of nitrogen-doped graphene quantum dots-Ru(bpy)32+-doped silica nanoparticles (SiO2@Ru-NGQDs). The ECL of SiO2@Ru-NGQDs was depressed when the distance between Ru(bpy)32+ and Fc was less than 8 nm; a stronger ECL was observed when the distance was more than 12 nm. The switching of the ECL of Ru(bpy)32+ by Fc was attributed to the electron transfer mechanism, in which Fc participated in the redox of Ru(bpy)32+ for “signal-off” ECL; this favored electron transfer at the electrode fabricated with an Fc-labeled aptamer (Fc-apt) and SiO2@Ru-NGQDs for “signal-on” ECL depending on the length of the DNA sequence. Here, a dual-signal readout aptasensor for aflatoxin B1 (AFB1) detection was developed via the enhanced ECL of SiO2@Ru-NGQDs by Fc-apt. The redox currents of Fc and the ECL of Ru(bpy)32+ were simultaneously collected as yardsticks, and both decreased with higher concentrations of AFB1. The aptasensor allowed linear ranges of 3 × 10–5 to 1 × 102 ng mL–1 for ECL mode and 1 × 10–3 to 3 × 103 ng mL–1 for electrochemical mode. Our work provides insight into the interactions between Fc and Ru(bpy)32+. The dual-signal readout strategy is a potential platform for the versatile design of aptasensors.
Funding Information
  • Jiangsu University
  • Natural Science Foundation of Jiangsu Province (BK20200104)
  • Government of Jiangsu Province (KYCX20_3087, NY-011)
  • National Natural Science Foundation of China (22074055, 61901193)
  • Priority Academic Program Development of Jiangsu Higher Education Institutions

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