Determination of telmisartan in rat tissues by in‐tube solid‐phase microextraction coupled to high performance liquid chromatography

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Abstract
A poly(methacrylic acid‐ethylene glycol dimethacrylate, MAA‐EGDMA) monolithic capillary was used for the direct and on‐line extraction of telmisartan from Sprague‐Dawley rat tissue (heart, kidney, and liver) homogenates. Under optimized conditions, the tissue homogenates were simply diluted with a mixture of phosphate buffer (pH 2)/ACN (90 : 8 v/v), and then injected for extraction only after centrifugation and filtration. Coupled to HPLC with fluorescence detection, the method was linear over the range of 1.25–1500 ng/g for telmisartan in heart and kidney, 12.5–15 000 ng/g in liver with correlation coefficients over 0.9992. The detection limits were found to be in the range from 0.24 to 1.8 ng/g. RSDs for intra‐ and inter‐day ranged from 1.2 to 8.1%. The determination of telmisartan in treated rat tissues was achieved by using the proposed method.

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