Lipoxygenase Inhibitory Activity of Octyl Gallate
Authors:
- 27 April 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 52 (10) , 3177-3181
Abstract
Octyl gallate inhibited soybean lipoxygenase-1 (EC 1.13.11.12, type I) with an IC50 of 1.3 μM. The inhibition of the enzyme by octyl gallate is a slow and reversible reaction without residual activity. The inhibition kinetics analyzed by Lineweaver−Burk plots indicates that octyl gallate is a competitive inhibitor, and the inhibition constant, KI, was obtained as 0.54 μM. One molecule of octyl gallate scavenged six molecules of 1,1-diphenyl-2-picrylhydrazyl and inhibited autoxidative lipid peroxidation. In addition, octyl gallate was effective in preventing lipid peroxidation. Keywords: Soybean lipoxygenase; octyl gallate; competitive inhibitor; Lineweaver−Burk plots; antioxidantKeywords
This publication has 17 references indexed in Scilit:
- Stopped-Flow Kinetic Investigations of the Activation of Soybean Lipoxygenase-1 and the Influence of Inhibitors on the Allosteric SiteBiochemistry, 2003
- Antifungal activity of octyl gallateInternational Journal of Food Microbiology, 2002
- Structure−Activity Relationship Studies of Nordihydroguaiaretic Acid Inhibitors toward Soybean, 12-Human, and 15-Human LipoxygenaseJournal of Medicinal Chemistry, 2002
- In Vitro Test for the Effectiveness of Antioxidants as Inhibitors of Thiyl Radical-Induced Reactions with Unsaturated Fatty AcidsJournal of Agricultural and Food Chemistry, 2001
- Antifungal activity of octyl gallate: structural criteria and mode of actionBioorganic & Medicinal Chemistry Letters, 2001
- Inhibition of Horse Bean and Germinated Barley Lipoxygenases by Some Phenolic CompoundsJournal of Food Science, 1995
- Prevention of Drug Access to Bacterial Targets: Permeability Barriers and Active EffluxScience, 1994
- Evaluation of the antioxidant and prooxidant actions of gallic acid and its derivativesJournal of Agricultural and Food Chemistry, 1993
- Chelating and free radical scavenging mechanisms of inhibitory action of rutin and quercetin in lipid peroxidationBiochemical Pharmacology, 1989
- NADPH-dependent microsomal lipid peroxidation and the problem of pathological action at a distanceBiochemical Pharmacology, 1978