Discovery of a New Male-sterile Allele, ms1-3, at MALE STERILITY 1 Locus in Cryptomeria japonica
Open Access
- 20 November 2025
- journal article
- Published by The Japanese Forest Society in Journal of the Japanese Forest Society
- Vol. 107 (11-12) , 256-261
Abstract
近年,スギ雄性不稔遺伝子MS1の候補遺伝子としてCJt020762が同定され,これまでに2つの雄性不稔対立遺伝子(ms1-1およびms1-2)が発見されている。本研究では,CJt020762のイントロンの一部を含めた全コード配列をPCR増幅して増幅断片の長さの違いを識別するPCRプライマーセット(DNAマーカー)を開発し,日本各地の育種素材1,500個体を対象に,挿入または欠失変異を持つ個体をマーカー選抜した。その結果,岩手県の育種素材で新たにコード配列に4塩基の欠失変異をヘテロ接合型で持つ2個体を選抜し,この対立遺伝子をms1-3と命名した。交配試験の結果,ms1-1およびms1-3をともに保有する個体(ms1-1/ms1-3)は雄性不稔であり,ms1-3が無花粉スギの育種に利用できることが示された。また本研究では,イントロン内に重複変異を生じた個体も認められたが,その個体は雄性不稔とならないことも示された。Keywords
Funding Information
- Bio-oriented Technology Research Advancement Institution (28013BC)
- Japan Society for the Promotion of Science (21K05666)
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