Fabrication and Characterization of Collagen‐Immobilized Porous PHBV/HA Nanocomposite Scaffolds for Bone Tissue Engineering

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Abstract
The porous composite scaffolds (PHBV/HA) consisting of poly (3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) (PHBV) and hydroxyapatite (HA) were fabricated using a hot‐press machine and salt‐leaching. Collagen (type I) was then immobilized on the surface of the porous PHBV/HA composite scaffolds to improve tissue compatibility. The structure and morphology of the collagen‐immobilized composite scaffolds (PHBV/HA/Col) were investigated using a scanning electron microscope (SEM), Fourier transform infrared (FTIR), and electron spectroscopy for chemical analysis (ESCA). The potential of the porous PHBV/HA/Col composite scaffolds for use as a bone scaffold was assessed by an experiment with osteoblast cells (MC3T3‐E1) in terms of cell adhesion, proliferation, and differentiation. The results showed that the PHBV/HA/Col composite scaffolds possess better cell adhesion and significantly higher proliferation and differentiation than the PHBV/HA composite scaffolds and the PHBV scaffolds. These results suggest that the PHBV/HA/Col composite scaffolds have a high potential for use in the field of bone regeneration and tissue engineering.
Funding Information
  • Ministry of Education, Science, and Technology of Korea (2010-0011125)

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