Functional CT for Quantifying Tumor Perfusion in Antiangiogenic Therapy in a Rat Model

Authors:
  • SPSith Phongkitkarun
    Sith Phongkitkarun
  • SKSatoshi Kobayashi
    Satoshi Kobayashi
  • YTYi Tang
    Yi Tang
  • LELee M. Ellis
    Lee M. Ellis
  • TLTing Y. Lee
    Ting Y. Lee
  • CCChusilp Charnsangavej
    Chusilp Charnsangavej
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Abstract
PURPOSE: To determine the histologic basis of perfusion parameters measured at functional computed tomography (CT) and to examine the relationship between changes in perfusion and changes in histologic parameters after antiangiogenic therapy in a rat model. MATERIALS AND METHODS: This study had institutional animal care and use committee approval. Among 20 Fischer rats with implanted FN13762 tumors in the liver, 10 were treated with SU5416, a tyrosine kinase inhibitor of vascular endothelial growth factor receptor, and 10 were treated with the diluent only as control rats. Six rats chosen at random from each group underwent functional CT for the measurement of tumor blood flow, blood volume, mean transit time, and permeability–surface area product. Tumor tissue slides corresponding to functional CT sections were examined to measure tumor microvascular density, number of luminal vessels, vascular perimeter, and vascular area. Two-tailed Student t testing was used to determine differences in growth, numbers...

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