Structural and dynamic similarities of nanofibrils and microparticles of engineered spider silk proteins probed by solid‐state NMR spectroscopy

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Abstract
Spider silks are proteinaceous fiber materials inspiring material design in various technical and biomedical fields due to their exceptional toughness, which exceeds that of most natural and artificial fibers. Solid‐state nuclear magnetic resonance (NMR) spectroscopy has been used herein to obtain insights into the structure and dynamics of 13 C/ 15 N isotope‐labeled nanofibrils and microparticles made of the recombinantly produced, engineered spider silk protein eADF4(C16), for which structural information was still lacking. Although these two β‐sheet‐rich morphologies differ substantially in their microscopic appearance (nanofibrils vs. microparticles), the solid‐state NMR spectra reveal high structural and dynamic similarities at the atomic level. For both morphologies, it was found that the rigid alanine stretch in the eADF4 sequence forms a mixture of rectangular and staggered β‐sheets extending to the flanking serine residues. In addition, our data reveal that the tyrosine sidechains are rigidified, which suggests their engagement in π–π‐stacking interactions. All of the glutamic acid residues were found to be deprotonated, which implies their localization on the outside of the fibril, where their negative charge can be compensated. Trans ‐ as well as cis ‐conformations were observed for proline residues, which suggests that they might further control the formation and extension of the poly‐alanine β‐sheet region during the self‐assembly process. The gained understanding of structure, dynamics, and assembly of the engineered spider silk protein eADF4(C16) will enable the tailored design of functional spider silk‐based biomaterials in the future. It will be especially useful in context of chemical modifications and genetic fusions supporting the development of fibril‐based hydrogel systems in the field of biosensing and tissue engineering.
Funding Information
  • Deutsche Forschungsgemeinschaft (448298270)
  • Deutsche Forschungsgemeinschaft (455238107)
  • Deutsche Forschungsgemeinschaft (455240421)

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