Glycoside hydrolases: Catalytic base/nucleophile diversity
Authors:
- 2 August 2010
- journal article
- review article
- Published by Wiley in Biotechnology and Bioengineering
- Vol. 107 (2) , 195-205
Abstract
Recent studies have shown that a number of glycoside hydrolase families do not follow the classical catalytic mechanisms, as they lack a typical catalytic base/nucleophile. A variety of mechanisms are used to replace this function, including substrate‐assisted catalysis, a network of several residues, and the use of non‐carboxylate residues or exogenous nucleophiles. Removal of the catalytic base/nucleophile by mutation can have a profound impact on substrate specificity, producing enzymes with completely new functions. Biotechnol. Bioeng. 2010;107: 195–205.Keywords
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