Expression of transforming growth factor-ß mRNA in experimental choroidal neovascularization

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Abstract
PURPOSE. Transforming growth factor beta (TGF-ß) is a multi-functional cytokine that modulates biological events as diverse as wound healing and angiogenesis and which may be important in the pathogenesis of choroidal neovascularization. We investigated the mRNA expression of TGF-ß isoforms in a model of experimental choroidal neovascularization induced by krypton-laser photocoagulation. METHODS. Rat TGF-ß1, mouse TGF-ß2 or TGF-ß3 cDNAs was inserted into the pBluescript vector to prepare antisense and sense riboprobes. Intense laser burns were applied to the posterior poles of the eyes of pigmented rats according to a protocol described for producing choroidal neovascularization in these animals. At intervals up to 4 weeks after photocoagulation, the eyes were obtained and cut into thin sections. The sections were subjected to in situ hybridization with digoxigenin (DIG)-labeled single-strand riboprobes synthesized from each TGF-ß cDNA. RESULTS. In normal adult rat retinas and choroids, TGF-ß1 mRNA was fo...

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