The effect of temperature on cold hardening ofLoliutn perenneseedlings

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Abstract
Summary: The variation in hardening responses under different temperature regimes for three cultivars ofLolium perenneL. is described. The relative cold hardiness of the cultivars was modified by different temperatures during hardening. A threshold temperature existed above which hardening did not occur, but this temperature varied between cultivars.Although continuous low temperatures (2 °C) favoured hardening, hardening also occurred under warm day and cool night conditions (15:2 °C) where cultivars showed contrasting hardiness responses in daylengths of 16, 12 and 8 h. Under some of these conditions both leaf growth and hardiness were possible.The significance of these results is discussed in terms of the development of screening techniques and breeding objectives.

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