ONT dA-tailing for Fungal Barcoding on MinION v2

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Abstract
This protocol is for dA-tailing, which is an enzymatic method for adding a non-templated nucleotide to the 3' end of a blunt, double-stranded DNA molecule. In other words, this puts a single adenine nucleotide at each 3′ end of our PCR product, creating a site for the ligation adapter to attach to. The module also repairs ends and provides 5′ phosphorylation. Simple process - create a reaction with three chemicals, cleanup the product with beads. Time required: ~45 minutes The NEB protocol this is based on can be found here.