First Report of Lasiodiplodia theobromae and Neofusicoccum parvum/N. ribis species Complex Causing Fruit Rot on Strawberry in the United States

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Abstract
During the last three seasons, approximately 1% of strawberry (Fragaria x ananassa) with symptoms of fruit softening and discoloration were observed in three fields in Florida. Fruit eventually became black and mummified with pycnidia embedded in the tissue that produced hyaline, aseptate conidia. Eleven isolates recovered from infected tissue were purified by growing them on 2% water agar (WA) with sterilized pine needles (Pavlic et al. 2004), and transferring a single conidium to a new 2% WA plate, by spreading a conidial suspension over the medium and selecting a single germinating conidium 16 h later. DNA of the 11 isolates was extracted from 2-week-old colonies and PCR was performed following the conditions of White et al. (1990) and Carbone and Kohn (1999). Two sets of universal primers, ITS1F/ITS4 and EF1-728F/EF1-986R, were used to amplify and sequence the internal transcriber spacer (ITS) and partial translation elongation factor 1-alpha (EF1-α) regions, respectively. Sequences compared to the Ge...

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