Fractionation of synaptophysin‐containing vesicles from rat brain and cultured PC12 pheochromocytoma cells
Authors:
- Hubert RehmHRHubert Rehm
- 21 November 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 240 (1-2) , 71-77
Abstract
Synaptophysin is a transmembrane glycoprotein of neuroendocrine vesicles. Its content and distribution in subcellular fractions from cultured PC12 cells, rat brain and bovine adrenal medulla were determined by a sensitive dot immunoassay. Synaptophysin‐containing fractions appeared as monodispersed populations similar to synaptic vesicles in density and size distribution. Membranes from synaptic vesicles contained ≈ 100‐times more synaptophysin than chromaffin granules. In conclusion, synaptophysin is located almost exclusively in vesicles of brain and PC12 cells which are distinct from dense core granules.Keywords
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