Identification of Bna.IAA7.C05 as allelic gene for dwarf mutant generated from tissue culture in oilseed rape

Authors:
Open Access
Publisher website
Google Scholar
Abstract
Background: Plant height is one of the most important agronomic traits in many crops due to its influence on lodging resistance and yield performance. Although progress has been made in the use of dwarfing genes in crop improvement, identification of new dwarf germplasm is still of significant interest for breeding varieties with increased yield.Results: Here we describe a dominant, dwarf mutant G7 ofBrassica napuswith down-curved leaves derived from tissue culture. To explore the genetic variation responsible for the dwarf phenotype, the mutant was crossed to a conventional line to develop a segregating F2population. Bulks were formed from plants with either dwarf or conventional plant height and subjected to high throughput sequencing analysis via mutation mapping (MutMap). The dwarf mutation was mapped to a 0.6 Mb interval ofB. napuschromosome C05. Candidate gene analysis revealed that one SNP causing an amino acid change in the domain II ofBna.IAA7.C05may contribute to the dwarf phenotype. This is consistent with the phenotype of a gain-of-functionindole-3-acetic acid(iaa) mutant inBna.IAA7.C05reported recently. GO and KEGG analysis of RNA-seq data revealed the down-regulation of auxin related genes, including many otherIAAandsmall up regulated response(SAUR) genes, in the dwarf mutant.Conclusion: Our studies characterize a new allele ofBna.IAA7.C05responsible for the dwarf mutant generated from tissue culture. This may provide a valuable genetic resource for breeding for lodging resistance and compact plant stature inB. napus.
Funding Information
  • Agricultural Science and Technology Innovation Project of Gansu Academy of Agricultural Sciences (2018GAAS04)
  • The Key program of Technological Innovation in Hubei Province (No. 2016ABA084)
  • The Earmarked Fund for Gansu Agriculture Research System
  • Open Research Fund of Key Laboratory for Biological Sciences and Genetic Improvement of Oil Crops, Ministry of Agriculture (KF2017010)
  • Fundamental Research Funds for Central Non-profit Scientific Institution (1610172017005)

This publication has 71 references indexed in Scilit: