ASSIGNMENT OF THE HUMAN PRO-ALPHA-2(I) COLLAGEN STRUCTURAL GENE (COLIA2) TO CHROMOSOME-7 BY MOLECULAR HYBRIDIZATION
Authors:
- C JUNIENCJC JUNIEN
- D WEILDWD WEIL
- JC MYERSJMJC MYERS
- N VANCONGNVN VANCONG
- ML CHUMCML CHU
- C FOUBERTCFC FOUBERT
- MS GROSSMGMS GROSS
- DJ PROCKOPDPDJ PROCKOP
- JC KAPLANJKJC KAPLAN
- F RAMIREZFRF RAMIREZ
- 1 January 1982
- journal article
- research article
- Vol. 34 (3) , 381-387
Abstract
A c[complementary]DNA for the pro.alpha.2 chain of human type I collagen was recently cloned and amplified. This specific probe was used to identify the human chromosome carrying the pro.alpha.2(I) collagen gene. The DNA from 17 independent human/hamster and human/mouse somatic cell hybrids was digested by Eco RI and the restriction pattern analyzed in Southern blot experiments, using the 32P-labeled cDNA as a hybridization probe. The gene coding for the pro.alpha.2 collagen subunit could be unambiguously assigned to human chromosome 7. All the other chromosomes, including chromosome 17, were excluded.Keywords
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